Monkey G-CSF ELISA kit

Sample

Serum, plasma, culture supernatant

Species Reactivity

Monkey

Detection Method

sELISA

Intended Use

The Monkey G-CSF ELISA kit allows for the quantitative detection of G-CSF in serum, plasma and culture supernatant.

Contents of Kit

RT Room temperature (temperature between 20 °C and 26 °C)
* Lyophilized
** For use in serum and plasma samples only, see section "Specimen Collection And Preparation"
*** Store protected from light

Storage

Coating and detection antibody
The vials with lyophilized coating and biotinylated detection antibody can be safely stored at 4 °C until the expiry date (indicated on the vials). After reconstitution, the antibodies are stable for at least 12 months at 4 °C when kept sterile. However, it is recommended to divide the reconstituted antibody solutions into small aliquots for single use. These aliquots should be stored at ≤-20 °C (stable for at least two years).
Standard
The vials with lyophilized standard can be safely stored at 4 °C until the expiry date (indicated on the vials). These vials are for single use only.
Conjugate
The vial with lyophilized SPP conjugate is stable until the expiry date (indicated on the vial) when stored protected from light at ≤-20 °C. After reconstitution, the reagent is stable for at least 2 months at 4 °C when kept sterile and protected from light. However, it is strongly recommended to divide the solution into small aliquots for single use. These aliquots should be stored protected from light at ≤-20 °C (stable for at least one year).
TMB
The ready-to-use TMB substrate solution should be stored at 4 °C and is stable until the expiry date (indicated on the vial). Avoid exposure to light, heat and contamination with metal ions or peroxidase.
BSA and Cytokine stabilization buffer
The vials with BSA stock solution and Cytokine stabilization buffer can be safely stored at 4 °C until the expiry date (indicated on the vial). After opening, these solutions are stable for at least 6 months when kept sterile.
Tween-20
Tween-20 can safely be stored at RT (protected from light) and is stable until the expiry date (indicated on the vial).
Stop solution
The ready-to-use stop solution can safely be stored at 4 °C and is stable until the expiry date (indicated on the vial).

Detection Range

2-128 pg/ml.

Sensitivity

2 pg/ml.

General Description

The ELISA is one of the primary and most popular methods to detect and measure signaling proteins like cytokines and granzymes. The ELISA test is rapid, simple to perform and is one of the most sensitive and reliable technologies available.

Standard Curve

Example of a representative Monkey G-CSF standard curve using a 4-parameter logistic fit.