Rabbit Anti-LDH monoclonal antibody, clone TV40-17

Linear alkylbenzene sulfonates (LAS) are widely detected in wastewater, and pose potential risks to environment. The influences of LAS on the typical pollutants removal in sequencing batch reactors (SBRs) were evaluated. The results indicated that the removal efficiency of COD, NH4+ and PO43- was respectively reduced by 10.5-27.5%, 5.4-7.3% and11.6-28.9% with the exposure of 10-50 mg/L LAS. Mechanisms investigation found that LAS disrupted the sludge structure and reduced the biomass in reactors due to the saponification effects. Also, the presence of LAS altered the microbial community of activated sludge, and reduced the abundances of functional bacterial responsible for pollutants removal (i.e.Candidatus Accumulibacter, Nitrospira, Denitratisoma and etc.). Moreover, the LAS exhibited negative impacts on the microbial activity with increased LDH release but decreased ATP concentration. The genes expressions for microbial metabolism (i.e. carbohydrate metabolisms, energy metabolism) and typical pollutants removal (i.e. electron transport, phosphonate transport) were all downregulated in LAS-exposed SBRs.

Cardiac abnormalities and dysfunction are the most important complications after renal ischemia-reperfusion (IR). Thus, investigation and development of effective treatment to decrease cardiac damage induced by renal ischemia are necessary. This study examined the effects of treatment with calcitriol and erythropoietin (EPO) on cardiac injury induced by renal ischemia. Wistar albino rats ere unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion. followed by 24 h reperfusion. Calcitriol and EPO were administered before ischemia. After 24 h reperfusion blood samples were collected for the determination of biochemical parameters. and kidney and cardiac samples were taken for histological studies. Renal IR increased BUN-Cr levels. lipid profiles. and myocardial injury markers (CK-MB and LDH). Histopathological findings of the IR group confirmed that there ere glomerular atrophy and acute tubular necrosis in the renal tissues and lymphocyte infiltration and intercellular edema in the cardiac samples. Treatment with calcitriol and EPO boosted cardiac and renal functions and improved the morphological changes. It seems that calcitriol or EPO administration could protect against the kidney and cardiac damage induced by IR. Also. the combination of calcitriol and EPO may exert more beneficial effects than either agent used alone.