Human TRIM28 blocking peptide

Name: Human TRIM28 blocking peptide
SKU: CDBP1662
Rating: 5 (1 reviews)

Synthetic Human TRIM28 blocking peptide for Apuri, BL, ELISA

COA

SDS

Datasheet

Summary

Product Overview

Blocking/Immunizing peptide for anti-KAP1/TRIM28 antibody

Target

KAP1 / TRIM28

Nature

Synthetic

Tag/Conjugate

Unconjugated

Application Notes

For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not for human or animal consumption.

Format

Lyophilized powder

Preservative

None

Storage

Shipped at ambient temperature, store at -20°C.

Entrez Gene ID

10155

Antigen Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Function

DNA binding; Krueppel-associated box domain binding; chromo shadow domain binding; poly(A) RNA binding; protein binding; protein kinase activity; contributes_to sequence-specific DNA binding; contributes_to sequence-specific DNA binding transcription fact

Synonyms

TRIM28; tripartite motif containing 28; KAP1; TF1B; RNF96; TIF1B; transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

TRIM28 is overexpressed in glioma and associated with tumor progression

ONCOTARGETS AND THERAPY

Authors: Su, Chunhai; Li, Hui; Gao, Wenbo

Abstract

Background: Tripartite motif containing 28 (TRIM28) is a transcriptional co-factor targeting many genes with pleiotropic biological activities, but the study on the role of TRIM28 in glioma is rare. Methods: To explore the function of TRIM28 in glioma, we first detected the expression levels of TRIM28 in glioma tissues and analyzed the correlations of TRIM28 expression with clinicopathological variables of patients in 85 cases of glioma. Meanwhile, we used shRNA to knockdown TRIM28 in glioma cell lines to detect the biological functions of TRIM28 in cell and animal experiments. Results: We found that TRIM28 was expressed at significantly higher level in glioma tissues than in non-tumor brain, and TRIM28 expression correlated significantly with tumor malignancy. Furthermore, TRIM28 higher expression was also correlated with poor survival of glioma patients (P<0.01). Functionally, knockdown of TRIM28 could significantly inhibit cell proliferation and migration in glioma cells. Additionally, we found that TRIM28 could inhibit the expression of E-cadherin significantly by reducing its mRNA stability at the post-transcriptional level. Conclusion: Our results suggest that TRIM28 overexpression is correlated with glioma malignant progression and patients' poor survival, so targeting TRIM28 could be an efficacious strategy in glioma.

Peri-conceptional under-nutrition alters the expression of TRIM28 and ZFP57 in the endometrium and embryos during peri-implantation period in domestic pigs

REPRODUCTION IN DOMESTIC ANIMALS

Authors: Zglejc, K.; Franczak, A.

Abstract

Contents DNA methylation is maintained by the main elements of methylation complextripartite motif containing 28 (TRIM28) and zinc finger protein 57 (ZFP57). Previously, it was found that the activity of TRIM28 and ZFP57 determines the process of DNA methylation and preserves over-expression of genes. We hypothesized that restricted diet applied during peri-conceptional period may induce changes in the expression of methylation complex in porcine endometrium and embryos during the peri-implantation period. The aim of this study was to detect and determine the expression of TRIM28 and ZFP57 in the endometrium and embryos harvested from gilts during the peri-implantation period (days 15-16 of pregnancy) fed restricted (n=5) or normal (n=5) diet during peri-conceptional period. In restricted-diet-fed gilts, endometrial expression of TRIM28 and ZFP57 mRNAs was decreased in comparison with normal-diet-fed gilts (p.01), while the embryonic expression of TRIM28 and ZFP57 mRNAs was increased in restricted-diet-fed gilts (p.05). The immunofluorescence showed the presence of TRIM28 and ZFP57 in luminal epithelial (LE), glandular epithelial (GE) and stromal cells (ST) of the endometrium as well as in the embryos. Total endometrial and embryonic abundance of TRIM28 and ZFP57 proteins was significantly higher (p.05) in restricted-diet-fed gilts than in normal-diet-fed gilts. Female under-nutrition during peri-conceptional period affects the expression of two main elements of methylation complex in the endometrium and in embryos during the peri-implantation period and may have the impact on DNA methylation in these tissues.