Species Reactivity
Human, Mouse, Rat
Intended Use
The JAK2 (Phospho-Tyr570) Colorimetric Cell-Based ELISA Kit allows for the qualitative detection of JAK2 (Phospho-Tyr570) and the effects that certain stimulation conditions have on JAK2 (Phospho-Tyr570) expression in different cell lines.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates.
2. 10x TBS: 24 mL (10x), Clear.
3. Quenching Buffer, 24 mL (1x), Clear.
4. Blocking Buffer, 50 mL (1x), Clear.
5. 10x Wash Buffer, 50 mL (10x), Clear.
6. 100x Anti-JAK2 (Phospho-Tyr570) Antibody (Rabbit Polyclonal) 60 μL (100x) Red.
7. 100x Anti-JAK2 Antibody (Rabbit Polyclonal) 60 μL (100x) Purple.
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 μL (100x) Green.
9. HRP-Conjugated Anti-Rabbit IgG Antibody, 12 mL (1x), Glass.
10. HRP-Conjugated Anti-Mouse IgG Antibody, 12 mL (1x), Glass.
11. Primary Antibody Diluent, 12 mL (1x), Clear.
12. Ready-to-Use Substrate, 12 mL (1x), Brown.
13. Stop Solution, 12 mL (1x), Clear.
14. Crystal Violet Solution, 12 mL (1x), Glass.
15. SDS Solution, 24 mL (1x), Clear.
16. Adhesive Plate Seals, 4 Seals.
Citations
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Liu, RY; Fan, C; et al. Constitutive activation of the JAK2/STAT5 signal transduction pathway correlates with growth factor independence of megakaryocytic leukemic cell lines. BLOOD 93:2369-2379(1999).
Ogata, N; Kouro, T; et al. JAK2 and JAK1 constitutively associate with an interleukin-5 (IL-5) receptor alpha and beta c subunit, respectively, and are activated upon IL-5 stimulation. BLOOD 91:2264-2271(1998).
COA
Certificate of Analysis
