A DNA sequence encoding the extracellular domain (Met 1-Gln 531) (28 Ser/Trp mutation) of the influenza hemagglutinin (A/Hong kong/213/2003 (H5N1)) (ABP51975.1) (HA1+HA2, cleavage site mutated RRRKKR-TETR, uncleaved), was expressed, fused with a C-terminal polyhistidine tag.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
The secreted recombinant influenza A H5N1 HA (A/Hong kong/213/03 (H5N1)) comprises 524 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H5N1; H5N1; IAV H5N1
Citations
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COA
Certificate of Analysis
