Human IL-17E/IL-25(Interleukin 25) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

In vitro quantitative determination of IL-17E/IL-25 concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	ELISA Microplate(Dismountable)	96T (8×12)	2-8°C/-20°C
2	Lyophilized Standard	2 vials	2-8°C/-20°C
3	Biotin-labeled Antibody	120ul	2-8°C
4	HRP-Streptavidin Conjugate (SABC, 100X)	120ul	2-8°C (Avoid Direct Light)
5	TMB Substrate	10ml	2-8°C (Avoid Direct Light)
6	Sample Dilution Buffer	20ml	2-8°C
7	Antibody Dilution Buffer	10ml	2-8°C
8	SABC Dilution Buffer	10ml	2-8°C
9	Stop Solution	10ml	2-8°C
10	Wash Buffer(25X)	30ml	2-8°C
11	Plate Sealer	5 pieces

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date.

Performance Characteristics

Stability: Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

15.625-1000pg/ml

Sensitivity

9.375pg/ml

Standard Curve