Intended Use
This ELISA kit is used for quantitative determination of obestatin in human plasma-EDTA samples.
Contents of Kit
Conjugate Solution (Streptavidin AChE Tracer)
Biotin-Labelled Antibody
Human Obestatin Standard
Quality Control
Dilution Buffer (EIA buffer)
Wash Solution
Substrate Solution (Ellman's Reagent)
Tween 20
Storage
Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Performance Characteristics
It is intended for research use only . The total assay time is about 24 h . The kit measures obestatin in EDTA plasma (K3EDTA)Assay format is 96 wells . Components of the kit are provided ready to use, concentrated or lyophilized.
Detection Range
31.3-4000 pg/ml
Detection Limit
62.5 pg/ml
General Description
Obestatin is an amidated peptide made of 23 amino-acids with a secondary conformation in alpha-helix. It was first isolated in 2005 from rat stomach. Obestatin is a preproghrelin-derived peptide and is produced by many tissues or organs like stomach, pancreas, adipose tissue, skeletal muscle or heart. Obestatin was identifi ed as an anorexigenic peptide with an action on the food intake. The first studies have shown that the obestatin reduced food intake and body weight. It has also been considered to be an antidiabetic peptide by positively influencing glucose and lipid metabolism.
Obestatin reduces the apoptosis and promote the proliferation of B-cells and human pancreatic islets. Due to heterogeneity of these sources, obestatin has many different functions. Indeed obestatin could have a function in the regulation of blood pressure and its plasmatic concentration increases in case of hypertensive patients. Finally obestatin could have a role in regulation of anxiety and improvement of the memory.
Citations
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