Human Ghrelin ( total ) ELISA Kit

Sample

Serum, plasma

Intended Use

This kit is used for the non-radioactive measurement of total human ghrelin ( both intact and des-octanoyl forms ) in serum and plasma.

Storage

All components of the kit should be stored at 4 °C. Prepare and use standard/QC solutions within a day after reconstitution and aliquot in smaller quantity and store at ≤ -20 °C for later use.

Detection Range

100 - 5000 pg/mL

Sensitivity

30 pg/mL

General Description

Ghrelin is a 28 amino acid hunger-stimulating peptide and hormone that is produced mainly by P/D1 cells lining the fundus of the
human stomach and epsilon cells of the pancreas. Ghrelin levels increase before meals and decrease after meals. It is considered the counterpart of the hormone leptin, produced by adipose tissue, which induces satiation when present at higher levels. In some bariatric procedures, the level of ghrelin is reduced in patients, thus causing satiation before it would normally occur. Ghrelin is a potent stimulator of growth hormone from the anterior pituitary gland. The ghrelin receptor is a G proteincoupled receptor, known as the growth hormone secretagogue receptor. Ghrelin binds to the GHSR1a splice-variant of this receptor which is present in high density in the hypothalamus, pituitary as well as vagal afferent cell bodies and vagal afferent endings throughout the gastro-intestinal tract Ghrelin plays a significant role in neurotrophy, particularly in the hippocampus, and is essential for cognitive adaptation to changing environments and the process of learning. Recently, ghrelin has been shown to activate the endothelial isoform of nitric oxide synthase in a pathway that depends on various kinases including Akt.