Human F11 Receptor, F11R ELISA Kit (CKERS-F11R-260H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5 plates
Species Reactivity
Human
Intended Use
The human F11R ELISA kit is for the quantitative determination of human F11R.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Capture Antibody: 0.5 mg/mL of mouse anti-F11R monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
2. Detection Antibody: Each vial contains 120μg biotinylated rabbit anti-F11R polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1.0 μg/mL in detection antibody dilution buffer before use.
3. Standard: Each vial contains 14ng of recombinant F11R. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°Cto -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 312.5 pg/mL is recommended.
4. Streptavidin-HRP: 50 μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.
Storage
Keep streptavidin-HRP at 4°Cand protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
4.88 pg/mL
Reconstitution And Storage
Bring all reagents to room temperature before use.
Capture Antibody: 0.5 mg/ml of mouse anti-F11R monoclonal antibody. Dilute to a working concentration of 2 μg/ml in CBS before coating.
Detection Antibody: Each vial contains 120μg biotinylated rabbit anti-F11R polyclonal antibody. Reconstitute with sterile 1ml distilled water. Dilute to a working concentration of 1.0μg/ml in detection antibody dilution buffer before use.
Standard: Each vial contains 14ng of recombinant F11R. Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20°Cto -70°Cin a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 312.5 pg/ml is recommended.
Streptavidin-HRP: 50μl of streptavidin conjugated to horse-radish-peroxidase. 1: 2, 000 Dilution in detection antibody dilution buffer before use.

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References


Azari, BM; Marmur, JD; et al. Transcription and translation of human F11R gene are required for an initial step of atherogenesis induced by inflammatory cytokines. JOURNAL OF TRANSLATIONAL MEDICINE 9:-(2011).
Naik, MU; Naik, UP; et al. Junctional adhesion molecule-A-induced endothelial cell migration on vitronectin is integrin alpha(v)beta(3) specific. JOURNAL OF CELL SCIENCE 119:490-499(2006).

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