Human Corticosterone, CORT ELISA Kit (CKERS-CORT-500H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
plasma, serum, urine, milk, saliva, cell culture supernatants
Species Reactivity
Human
Intended Use
Quantitative Determination of corticosterone in plasma, serum, urine, milk, saliva, and cell culture supernatant.
Contents of Kit
1. Corticosterone Microplate: A 96 well polystyrene microplate(12 strips of 8 wells) coated with a polyclonal antibody against Corticosterone.
2. Sealing Tapes: Each kit contains 3pre-cut, pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
3. Corticosterone Standard: Corticosterone in abuffered protein base(400 ng/mL, 0.5 mL).
4. Biotinylated Corticosterone: 1 vial,lyophilized.
5. EIA Diluent Concentrate(10x): A 10-fold concentrated buffered protein base(20 mL).
6. Wash Buffer Concentrate(20x): A 20-fold concentrated buffered surfactant(30 mL, 1 bottle).
7. Streptavidin-Peroxidase Conjugate (SP Conjugate, 100x): A100-fold concentrate(80 μL).
8. Chromogen Substrate: A ready-to-usestabilized peroxidase chromogen substrate tetramethylbenzidine(8 mL).
9. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction(12 mL).
Storage
1.Store components of the kit at 2-8°C or -20°C upon arrival up to theexpiration date.
2.Store Standard and SP Conjugate at -20°C.
3.Store Microplate, Diluent Concentrate (10x), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8°C.
4.Opened unused microplate wells may be returned to the foil pouch with thedesiccant packs. Reseal along zip-seal. May be stored for up to 1 month in avacuum desiccator.
5.Diluent (1x) may be stored for up to 1 month at 2-8°C.
6.Store Biotinylated Protein at 2-8°C before reconstituting with Diluent andat -20°C after reconstituting with Diluent.
Performance Characteristics
1.The minimum detectable dose of Corticosterone is typically 0.3 ng/mL.
2.Intra-assay and inter-assay coefficients of variation were 5.0 % and 7.2 % respectively.
Standard Curve

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