Corticosterone ELISA Kit

Name: Corticosterone ELISA Kit
SKU: DEIABL244
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, EDTA and Heparin Plasma, Saliva, Urine, Dried Fecal Extracts, and Tissue Culture Media

Intended Use

The Corticosterone ELISA Kit, with its adaptable format and high sensitivity, is a valuable tool for research in endocrinology, stress physiology, and neurobiology. It provides precise measurement of corticosterone levels, aiding in the study of its effects on physiological and behavioral responses.

Contents of Kit

1. Coated Clear 96 Well Plates:
A clear plastic microtiter plate(s) coated with donkey anti-sheep IgG.
2. Corticosterone Standard:
Corticosterone at 100,000 pg/mL in a special stabilizing solution. 125 μL
3. Corticosterone Antibody:
A sheep polyclonal antibody speciffc for corticosterone. 3 mL
4. Corticosterone Conjugate:
A corticosterone-peroxidase conjugate in a special stabilizing solution. 3 mL
5. Assay Buffer Concentrate:
A 5× concentrate that must be diluted with deionized or distilled water. 28 mL
6. Dissociation Reagent:
Dissociation Reagent is to be used only with Serum and Plasma samples. 1 mL
7. Wash Buffer Concentrate:
A 20× concentrate that should be diluted with deionized or distilled water. 30 mL
8. TMB Substrate:
11 mL
9. Stop Solution:
A 1M solution of hydrochloric acid. CAUSTIC. 5 mL
10. Plate Sealer:
1

Storage

All components of this kit should be stored at 4°C until the expiration date of the kit.

Precision

Intra Assay Precision - 50 μL Assay Format
Four human samples were diluted with 1× Assay Buffer and run in replicates of 20 in an assay. The mean and
precision of the calculated Corticosterone concentrations were:

Inter Assay Precision - 50 μL Assay Format
Three human samples were diluted with 1× Assay Buffer and run in duplicates in fourteen assays run over multiple days by four operators. The mean and precision of the calculated Corticosterone concentrations were:

Sensitivity

Sensitivity was calculated by comparing the OD's for twenty wells run for each of the B0 and standard #9. The detection limit was determined at two (2) standard deviations from the B0 along the standard curve. Sensitivity was determined as 20.9 pg/mL for 50 μL and 14.35 pg/mL for 100 μL sample size.
The Limit of Detection for the assay was determined in a similar manner by comparing the OD's for twenty runs for each of the zero standard and a low concentration human sample. Limit of Detection was determined as 17.5 pg/mL for 50 μL and 7.7 pg/mL for 100 μL sample size.

General Description

Corticosterone (C₂₁H₃₀O₄, Kendall's Compound 'B') is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.

Standard Curve

Always run your own standard curve for calculation of results. Do not use this data.
Conversion Factor: 100 pg/mL of corticosterone is equivalent to 288.6 pM.
Typical Normal Range Standard Curves

Always run your own standard curves for calculation of results. Do not use this data.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Baseline corticosterone does not reflect iridescent plumage traits in female tree swallows

GENERAL AND COMPARATIVE ENDOCRINOLOGY

Authors: Sarpong, Keneth; Madliger, Christine L.; Harris, Christopher M.; Love, Oliver P.; Doucet, Stephanie M.; Bitton, Pierre-Paul

Abstract

The production of high quality secondary sexual traits can be constrained by trade-offs in the allocation of energy and nutrients with other metabolic activities, and is mediated by physiological processes. In birds, the factors influencing male plumage quality have been well studied; however, factors affecting female plumage quality are poorly understood. Furthermore, it remains uncertain which physiological traits mediate the relationship between body condition and ornaments. In this three-year study of after-second-year female tree swallows (Tachycineta bicolor), we investigated (1) the relationship between baseline corticosterone near the end of the brood-rearing period (CORTBR) and feather colour characteristics (hue, saturation, brightness) the following year, and (2) the relationship between baseline corticosterone measured during incubation (CORTI) and brood rearing (CORTBR), and feather colour in the same year. To control for reproductive effort, we included reproductive parameters as covariates in all analyses. In this first study between CORT and the plumage colour characteristics of a species bearing iridescent feathers, we did not find any relationship between CORTBR and the colour of subsequently-produced feathers, nor did we find any relationship between CORT and the colour of feathers displayed during that breeding season. If CORT levels at the end of breeding carry over to influence the immediately subsequent moult period as we expect, our results generally indicate that structural plumage quality may not be as sensitive to circulating CORT levels compared to carotenoid-based colouration. Future studies, particularly those employing experimental manipulations of CORT during moult in species with iridescent traits, are necessary to fully determine the role glucocorticoids play in mediating the quality of secondary sexual characteristics.

Pre-weaning paternal deprivation impairs social recognition and alters hippocampal neurogenesis and spine density in adult mandarin voles

NEUROBIOLOGY OF LEARNING AND MEMORY

Authors: He, Zhixiong; Guo, Qianqian; Yang, Yang; Wang, Limin; Zhang, Siyi; Yuan, Wei; Li, Laifu; Zhang, Jing; Hou, Wenjuan; Yang, Jinfeng; Jia, Rui; Tai, Fadao

Abstract

Disruption of the early social environment, such as maternal separation or early deprivation, can impair cognitive function, alter offspring neurogenesis and restrict dendritic architecture in the hippocampus. However, whether paternal deprivation during the pre-weaning period affects adult neurogenesis, synaptogenesis and social recognition remains unclear in monogamous species. In the present study, mandarin vole (Microtus mandarinus) pups were deprived of fathers during postnatal day 14-21. Then social recognition, hippocampal neurogenesis and spine density, basal levels of corticosterone (CORT) and oxytocin (OT) were examined at adulthood. We found that paternal deprivation impaired social recognition at adulthood. In addition, paternal deprivation significantly reduced 5-bromo-2-deoxyuidine (BrdU) immunoreactive cells (p < 0.01) and Brdu/Neun-labeled cells (p < 0.05) in the dentate gyrus compared to those of biparental care group in females, but not in males (p > 0.05). Meanwhile, paternal deprivation group had fewer double-staining cells with BrdU and the immature neuron marker doublecortin than biparental care group both in male (p < 0.01) and female (p < 0.05) voles. Paternal deprivation also decreased the number of dendritic spines in the dentate gyrus at adulthood. Paternal deprivation reduced circulating levels of OT and increased CORT only in females. These results demonstrated that impaired social recognition induced by paternal deprivation may be linked with alterations in neurogenesis and spine density of the dentate gyrus and levels of OT and CORT, especially in females.