Anti-CORT polyclonal antibody [Biotin] (DPABH-16126)

Rabbit anti-Human CORT (aa 20-40) polyclonal antibody for RIA, ELISA

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Synthetic peptide: CSALPLESGPTGQDSVQDATG conjugated to KLH, corresponding to amino acids 20-40 of Human Cortistatin.
Conjugate
Biotin

Target


Alternative Names
CORT; cortistatin; CST-14; CST-17; CST-29; cortistatin-14
Entrez Gene ID
UniProt ID

Product Background


Pathway
Cell Cycle; M Phase; Mitotic Metaphase and Anaphase; Resolution of Sister Chromatid Cohesion

Citations


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References


Influence of Low Protein Diet-Induced Fetal Growth Restriction on the Neuroplacental Corticosterone Axis in the Rat

FRONTIERS IN ENDOCRINOLOGY

Authors: Schmidt, Marius; Rauh, Manfred; Schmid, Matthias C.; Huebner, Hanna; Ruebner, Matthias; Wachtveitl, Rainer; Cordasic, Nada; Rascher, Wolfgang; Menendez-Castro, Carlos; Hartner, Andrea; Fahlbusch, Fabian B.

Objectives: Placental steroid metabolism is linked to the fetal hypothalamus-pituitary-adrenal axis. Intrauterine growth restriction (IUGR) might alter this cross-talk and lead to maternal stress, in turn contributing to the pathogenesis of anxiety-related disorders of the offspring, which might be mediated by fetal overexposure to, or a reduced local enzymatic protection against maternal glucocorticoids. So far, direct evidence of altered levels of circulating/local glucocorticoids is scarce. Liquid chromatography tandem-mass spectrometry (LC-MS/MS) allows quantitative endocrine assessment of blood and tissue. Using a rat model of maternal protein restriction (low protein [LP] vs. normal protein [NP]) to induce IUGR, we analyzed fetal and maternal steroid levels via LC-MS/MS along with the local expression of 11beta-hydroxysteroid- dehydrogenase (Hsd11b). Methods: Pregnant Wistar dams were fed a low protein (8%, LP; IUGR) or an isocaloric normal protein diet (17%, NP; controls). At E18.5, the expression of Hsd11b1 and 2 was determined by RT-PCR in fetal placenta and brain. Steroid profiling of maternal and fetal whole blood, fetal brain, and placenta was performed via LC-MS/MS. Results: In animals with LP-induced reduced body (p < 0.001) and placental weights (p < 0.05) we did not observe any difference in the expressional Hsd11b1/2-ratio in brain or placenta. Moreover, LP diet did not alter corticosterone (Cort) or 11-dehydrocorticosterone (DH-Cort) levels in dams, while fetal whole blood levels of Cort were significantly lower in the LP group (p < 0.001) and concomitantly in LP brain (p = 0.003) and LP placenta (p = 0.002). Maternal and fetal progesterone levels (whole blood and tissue) were not influenced by LP diet. Conclusion: Various rat models of intrauterine stress show profound alterations in placental Hsd11b2 gatekeeper function and fetal overexposure to corticosterone. In contrast, LP diet in our model induced IUGR without altering maternal steroid levels or placental enzymatic glucocorticoid barrier function. In fact, IUGR offspring showed significantly reduced levels of circulating and local corticosterone. Thus, our LP model might not represent a genuine model of intrauterine stress. Hypothetically, the observed changes might reflect a fetal attempt to maintain anabolic conditions in the light of protein restriction to sustain regular brain development. This may contribute to fetal origins of later neurodevelopmental sequelae.

Maternal-induced shifts in allostatic demands: Reproductive experience alters emotional and cognitive biobehavioral responses in rats (Rattus norvegicus)

NEUROSCIENCE LETTERS

Authors: Sullivan, Emily D. K.; Kent, Molly; Thompson, Brooke; Bardi, Massi; Lambert, Kelly

The arrival and subsequent care of offspring require abrupt shifts in biobehavioral responses in mammalian mothers. In the current study, female rats with one reproductive experience [primiparous (PRIM) rats, n = 8] or no reproductive experience [nulliparous (NULL) rats, n = 8] were assessed in a dry land maze to determine both learning acquisition and responses to uncertainty/prediction errors during the probe trial. Additionally, rats were observed in a swim task and an open field arena to assess responsiveness to varied environmental challenges. Results indicated that the PRIM rats investigated more previously baited wells during the probe trial (ontask behavior) whereas the NULL rats exhibited more peripheral-oriented rearing responses (off-task behavior). Further, a nonsignificant trend was observed indicating more dive responses in the PRIM animals. Focusing on endocrine markers, the PRIM animals had higher DHEA/CORT ratios than the NULL animals following the probe trial. Finally, PRIM animals had less hippocampal glucocorticoid receptor immunoreactivity and more hippocampal BDNF immunoreactivity than NULL animals. In sum, behavioral, endocrine and neural markers suggest that PRIM rats exhibit long-lasting modifications to stress responsivity.

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