Human Carcinoembryonic Antigen-related Cell Adhesion Molecule 6 (non-specific cross reacting antigen), CEACAM6 ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human CEACAM6 ELISA kit is for the quantitative determination of human CEACAM6.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Capture Antibody: 0.5 mg/mL of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
2. Detection Antibody: Each vial contains 140 μg biotinylated rabbit anti-CEACAM6 polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 2 μg/mL in detection antibody dilution buffer before use.
3. Standard: Each vial contains 16 ng of recombinant CEACAM6. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1, 000 ng/mL is recommended.
4. Streptavidin-HRP: 50 μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.

Storage

Keep streptavidin-HRP at 4°C and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

15.6 pg/mL

Reconstitution And Storage

Bring all reagents to room temperature before use.
##Capture Antibody: ##0.5 mg/ml of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2 μg/ml in CBS before coating.
##Detection Antibody: ##Each vial contains 140ug biotinylated rabbit anti-CEACAM6 polyclonal antibody. Reconstitute with sterile 1ml distilled water. Dilute to a working concentration of 2 μg/ml in detection antibody dilution buffer before use.
##Standard: ##Each vial contains 16ng of recombinant CEACAM6. Reconstitute with 1 ml detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1, 000 ng/ml is recommended.
##Streptavidin-HRP: ##50μl of streptavidin conjugated to horse-radish-peroxidase. 1: 2, 000 Dilution in detection antibody dilution buffer before use.