Human Cadherin 1, Type 1, E-cadherin (epithelial), CDH1 ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human CDH1 ELISA kit is for the quantitative determination of human CDH1.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Capture Antibody: 0.4 mg/mL of mouse anti-CDH1 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
2. Detection Antibody: 0.5 mg/mL of biotinylated rabbit anti-CDH1 polyclonal antibody. Dilute to a working concentration of 0.3 μg/mL in detection antibody dilution buffer before use.
3. Standard: Each vial contains 100ng of recombinant CDH1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
4. Streptavidin-HRP: 50 μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.

Storage

Keep streptavidin-HRP at 4°Cand protect it from pro-longed exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

46.8 pg/mL

Reconstitution And Storage

Bring all reagents to room temperature before use.
##Capture Antibody: ##0.4 mg/ml of mouse anti-CDH1 monoclonal antibody. Dilute to a working concentration of 2 μg/ml in CBS before coating.
##Detection Antibody: ##0.5 mg/ml of biotinylated rabbit anti-CDH1 polyclonal antibody. Dilute to a working concentration of 0.3 μg/ml in detection antibody dilution buffer before use.
##Standard: ##Each vial contains 100ng of recombinant CDH1. Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/ml is recommended.
##Streptavidin-HRP: ##50μl of streptavidin conjugated to horse-radish-peroxidase. 1: 2, 000 Dilution in detection antibody dilution buffer before use.