Human Bone Marrow Stromal Cell Antigen 1, BST1 ELISA Kit

Name: Human Bone Marrow Stromal Cell Antigen 1, BST1 ELISA Kit
SKU: DEIA233
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Plasma, tissue homogenates and other biological fluids.

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Human CD157/BST1 ELISA Pair Set is for the quantitative determination of Human CD157/BST1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

Contents of Kit

Capture Antibody: 0.3 mg/mL of mouse anti-Human CD157/BST1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Antibody: 0.2 mg/mL of rabbit anti-Human CD157/BST1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard: Each vial contains 38 ng of recombinant Human CD157/BST1. Reconstitute with 1mL detection antibody dilution buffer. After reconstitution, store at -20°C to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.

Storage

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. Do not freeze!
Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted standard at -80°C for up to 1 month. Avoid repeated freezethaw cycles.

Sensitivity

The minimum detectable dose of Human CD157/BST1 was determined to be approximately 23.44 pg/ml.
This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

General Description

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2− ) production in the human U937 myeloid line.

Standard Curve

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Documents

pdf COA

pdf SDS

pdf Datasheet

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References

Association Study between the CD157/BST1 Gene and Autism Spectrum Disorders in a Japanese Population

BRAIN SCIENCES

Authors: Yokoyama, Shigeru; Al Mahmuda, Naila; Munesue, Toshio; Hayashi, Kenshi; Yagi, Kunimasa; Yamagishi, Masakazu; Higashida, Haruhiro

Abstract

CD157, also referred to as bone marrow stromal cell antigen-1 (BST-1), is a glycosylphosphatidylinositol-anchored molecule that promotes pre-B-cell growth. Previous studies have reported associations between single-nucleotide polymorphisms (SNPs) of the CD157/BST1 gene with Parkinson's disease. In an attempt to determine whether SNPs or haplotypes in the CD157/BST1 are associated with other brain disorders, we performed a case-control study including 147 autism spectrum disorder (ASD) patients at Kanazawa University Hospital in Japan and 150 unselected Japanese volunteers by the sequence-specific primer-polymerase chain reaction method combined with fluorescence correlation spectroscopy. Of 93 SNPs examined, two SNPs showed significantly higher allele frequencies in cases with ASDs than in unaffected controls (rs4301112, OR = 6.4, 95% CI = 1.9 to 22, p = 0.0007; and rs28532698, OR = 6.2, 95% CI = 1.8 to 21, p = 0.0012; Fisher's exact test; p < 0.002 was considered significant after multiple testing correction). In addition, CT genotype in rs10001565 was more frequently observed in the ASD group than in the control group (OR = 15, 95% CI = 2.0 to 117, p = 0.0007; Fisher's exact test). The present data indicate that genetic variation of the CD157/BST1 gene might confer susceptibility to ASDs.

Analysis of Genome-wide Association Study-linked Loci in Parkinson's Disease of Mainland China

MOVEMENT DISORDERS

Authors: Liu, Jun; Xiao, Qin; Wang, Ying; Xu, Zhi-Min; Wang, Ying; Yang, Qiong; Wang, Gang; Tan, Yu-Yan; Ma, Jian-Fang; Zhang, Jin; Huang, Wei; Chen, Sheng-Di

Abstract

BackgroundGenome-wide association studies (GWAS) have identified numerous single-nucleotide polymorphisms (SNPs) that can modulate the risk of developing Parkinson's disease (PD). MethodsWe investigated the association of previously identified loci in a Mainland Chinese population to identify a possible ethnic-specific effect with GWAS analysis. Seventeen SNPs were genotyped from those loci using case-control methodology to analyze a total of 1,737 individuals. ResultsStrong evidence of an association for reference SNP 894278 (rs894278) and rs11931074 on 4q22 throughout the synuclein (SNCA) region was observed in our study. The SNP rs894278 confers risk via a dominant model and an additive model, whereas the minor allele G of rs11931074 reduces the risk of PD progression. The minor allele frequency of rs11724635 produced weaker signals for PD, but this was not replicated in the genotype after adjusting for age and sex. ConclusionsThis study yields new clues about GWAS-linked data in patients with PD from Mainland China. (c) 2013 International Parkinson and Movement Disorder Society