Human Bone Marrow Stromal Cell Antigen 1, BST1 ELISA Kit (CKERS-BST1-229H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5 plates
Species Reactivity
Human
Intended Use
The human BST1 ELISA kit is for the quantitative determination of human BST1.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Capture Antibody: 0.5 mg/mL of mouse anti-BST1 monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
2. Detection Antibody: 0.3 mg/mL of biotinylated rabbit anti-BST1 polyclonal antibody. Dilute to a working concentration of 0.2 μg/mL in detection antibody dilution buffer before use.
3. Standard: Each vial contains 15 ng of recombinant BST1. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
4. Streptavidin-HRP: 50 μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.
Storage
Keep streptavidin-HRP at 4°Cand protect it from pro-longed exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
Sensitivity
15.6 pg/mL
Reconstitution And Storage
Bring all reagents to room temperature before use.
Capture Antibody: 0.5 mg/ml of mouse anti-BST1 monoclonal antibody. Dilute to a working concentration of 1 μg/ml in CBS before coating.
Detection Antibody: 0.3 mg/ml of biotinylated rabbit anti-BST1 polyclonal antibody. Dilute to a working concentration of 0.2 μg/ml in detection antibody dilution buffer before use.
Standard: Each vial contains 15 ng of recombinant BST1. Reconstitute with 1 ml detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/ml is recommended.
Streptavidin-HRP: 50μl of streptavidin conjugated to horse-radish-peroxidase. 1: 2,000 Dilution in detection antibody dilution buffer before use.

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