Hemoglobin/Haptoglobin-Complex ELISA Kit

Sample

Stool

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Hemoglobin/Haptoglobin Complex ELISA is intended for the quantitative determination of hemoglobin/haptoglobin complex in human stool samples. For Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1.Hemoglobin/Haptoglobin Microplate (96 wells), 12 strips of 8 wells, Ready to use
2.Standards (1-5) (0, 0.67, 3.3, 10, 50 μg/g), 2 x 5 vials, Lyophilized
3.Control Levels 1 and 2, 2 vials each, Lyophilized
4.Wash Buffer Concentrate, 2 x 100 mL, 10X
5.Extraction Buffer Concentrate, 1 x 100 mL, 2.5X
6.Conjugate, 1 x 15 mL, Ready to use
7.Sample Dilution Buffer, 2 x 15 mL, Ready to use
8.TMB Substrate, 15 mL, Ready to use
9.Stop Solution, 15 mL, Ready to use

Storage

The kit should be stored at 2-8°C. The kit is stable until the expiration date on the box label.

Precision

Repeatability (Intra-Assay)
The repeatability was assessed with 2 stool samples under constant parameters (same operator, measurement system, day and kit lot).

Reproducibility (Inter-Assay)
The reproducibility was assessed with 2 control samples under varying parameters (different operators, measurement systems, days, and kit lots).

Sensitivity

Analytical Sensitivity
The following values have been estimated based on the concentrations of the standard without considering possibly used sample dilution factors:
Limit of Blank, (LoB) 0.033 μg/g
Limit of Detection (LoD) 0.158 μg/g
Limit of Quantitation (LoQ) 0.248 μg/g
The specified accuracy goal for the LoQ was 20% CV.