Recombinant HIV type 1 Nef Antigen (a.a. 3-190) [BGAL]

Product Overview

Recombinant Human Immunodeficiency Virus Type 1 Nef Antigen Strain IIIB (a.a. 3-190). HIV-I nef antigen a.a 3 to a.a 190 of the HIV-I nef region. 20kDa with Beta-galactosidase (114 kDa) fused at the N-terminus. Reactive with human HIV positive serum. It w

Nature

Recombinant

Tag/Conjugate

Beta-galactosidase

Molecular Weight

1340 kDa

Alternative Names

Human Immunodeficiency Virus; HIV Nef; HIV; HIV-1 Nef; HIV-1; HIV type 1 Nef

Purity

> 95% pure (SDS-PAGE)

Format

Liquid

Concentration

1 mg/ml (OD280nm)

Buffer

8M Urea, 20mM Tris-HCl, pH 8.0; 10mM Beta-mercaptoethanol

Preservative

None

Storage

Short-term (up to 2 months) store at 2–8°C. Long term, aliquot and store at -80°C. Avoid multiple freeze/thaw cycles.

Introduction

HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.

Keywords

Human Immunodeficiency Virus; HIV Nef; HIV; HIV-1 Nef; HIV-1; HIV type 1 Nef