HIV type 1 Nef, recombinant protein from E. coli
HIV1 Nef protein fragment
Reacts strongly with human HIV positive serum.
> 95 % by SDS-PAGE.
Preservative: None Constituents: 0.014M Beta mercaptoethanol, 0.01M Sodium carbonate, 0.01M EDTA
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. Preservative: None Constituents: 0.014M Beta mercaptoethanol, 0.01M Sodium carbonate, 0.01M EDTA
The human immunodeficiency virus (HIV) is a lentivirus (slowly replicating retrovirus) that causes the acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
C terminal core protein; F protein; Nef; Negative factor; p27; HIV1 Nef