The test can be used to detect GLRaV-5 in host plants.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is relative high. The virus can be consistently detected in infected plant tissues diluted at 1:810 -1:2430.
General Description
Grapevine leafroll-associated virus (GLRaV) is a name for a group of viruses infecting grapevine in the genus Closterovirus. Obscure mealybugs (Pseudococcus viburni) feed on the phloem of vines and woody-stemmed plants, especially pear and apple trees and grape vines. Some individuals are vectors for infectious pathogens and can transmit them from plant to plant while feeding; mealybug-spread grapevine leafroll associated virus type III (GRLaV-3), in particular, has wreaked havoc among the grapes of New Zealand, reducing the crop yield of infected vineyards by up to 60%. Leafroll viruses are associated with rugose wood condition of grapevine.
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