Grapevine Leafroll Associated Virus 1 ELISA Development Kit

Viticulture is important in Argentina's economy, especially in the province of Mendoza, which is responsible for more than 75% of the crop cultivated area. In this work, we evaluated the incidence of Grapevine leafroll-associated viruses (GLRaV) -1, -2, and -3 in Vitis vinifera clones of cultivars Cabernet Sauvignon, Cabernet Franc, and Sauvignon Blanc, planted in different zones of Mendoza. The selected clones were previously reported as putatively infected by GLRaV-2. All selected samples were analyzed by DAS-ELISA for GLRaV-1,-2 and -3. GLRaV-2 was the only virus identified in all the analyzed clones. The overall infection rates were 0.6%, 18.8% and 1.2% for GLRaV-1, 2 and 3 respectively. For the clone Cabernet Sauvignon 337, the infection rate was very high (68.3%).

We have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.