The Elk1 ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor target protein Elk1 and expression profile in cells. The kit can be used for measuring the relative amounts of Elk1 proteins in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Elk1.
Contents of Kit
No.
Components
Size
Storage Conditions
1
96-Well Microplate
1 Plate
4°C
2
10× TBS
30 ml
4°C
3
Quenching Buffer
24 ml
4°C
4
Blocking Buffer
50 ml
4°C
5
15× Wash Buffer
60 ml
4°C
6
100× Anti-Elk1 Antibody
60 μl
4°C
7
100× Anti-GAPDH Antibody
60 μl
4°C
9
HRP-Conjugated Anti-Rabbit IgG Antibody
6 ml
4°C
10
HRP-Conjugated Anti-Mouse IgG Antibody
6 ml
4°C
11
Primary Antibody Diluent
12 ml
4°C
12
Ready-to-Use Substrate
12 ml
4°C
13
Stop Solution
12 ml
4°C
14
Crystal Violet Solution
6 ml
4°C
15
SDS Solution
24 ml
4°C
16
Adhesive Plate Sealers
2 Seals
Storage
Store whole kit at 4 °C for 6 months from receipt.
Citations
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