Anti-CK-MM polyclonal antibody

Objective: To observe the effect of An-pressing manipulation on biceps brachii with delayed onset muscle soreness (DOMS) in healthy male volunteers. Methods: A total of 30 male college student volunteers were randomly divided into a blank group, a model group and a treatment group, 10 cases in each group. Subjects in the blank group did not receive any intervention; subjects in the model group received active weight-bearing eccentric exercise on the non-favored side of the upper limb to establish the models, while not receiving any treatment; subjects in the treatment group received both the same modeling and An-pressing manipulation treatment. The subjective rating of perceived exertion (RPE), subjective soreness sensation threshold and soreness grade were evaluated before modeling, immediately after modeling, and 24, 48, 72, 96 and 120 h after modeling. Serum total antioxidant capacity (T-AOC) was measured before modeling, immediately after modeling, and 24, 48 and 72 h after modeling. Serum creatine kinase MM isoenzyme (CK-MM) was measured before modeling and 24, 48 and 72 h after modeling. Results: At 24, 48, 72 and 120 h after treatment, the soreness grades of the treatment group were lower than those of the model group (all P < 0.05). The RPE scores of the treatment group were lower than those of the model group (all P < 0.05) immediately after modeling, at 24, 48, 72, 96 and 120 h after modeling. The subjective soreness sensation threshold of the treatment group was higher than that of the model group immediately after modeling, at 24, 48, 72 and 96 h after modeling (all P < 0.05). Immediately after modeling, T-AOC value in the treatment group was higher than that in the model group and blank group (both P < 0.05). CK-MM of the treatment group was lower than that of the model group at 48 h and 72 h after modeling (P < 0.05). Conclusion: An-pressing manipulation shows a certain therapeutic effect on biceps brachii with DOMS by strengthening the body's antioxidant and anti-damage abilities, which can effectively reduce the pain and accelerate the recovery from fatigue damage.

AimTubal ectopic pregnancy (tEP) must be diagnosed as early as possible because it is one of the leading causes of pregnancy-related death in the first trimester. The aim of this study was to evaluate the diagnostic value of serum total creatine kinase (CK) and its isoforms in diagnosis of tEP. MethodsThe study included 32 tEP cases, and gestational age-matched intrauterine (IU) abortion and normal pregnancy cases (n=31 each). Serum total CK and CK-MB fraction were measured by the principle of spectrophotometry and CK-MM fraction was calculated from the above parameters. Serum -hCG was measured using chemiluminescent immunoassay. All study parameters were measured at the time of presentation. ResultsSerum -hCG levels were significantly low in patients with both tEP and IU abortion than normal pregnancy. Women with tEP had higher concentrations of total CK, CK-MM and lower concentrations of CK-MB% compared to both gestational age-matched IU pregnancy controls and IU abortion. Receiver-operator characteristic analysis revealed that the optimal cut-off for total CK, CK-MM and CPK-MB% as predictors of ruptured EP were 147IU/L, 135IU/L and 10%, respectively, with the former two having higher specificity, and latter high sensitivity. ConclusionEstimation of CK and its CK isoenzyme fractions can aid in quick and accurate diagnosis of tEP.