Species Reactivity
Human, Mouse
Detection Method
Indirect ELISA
Intended Use
The CEBPB Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CEBPB protein expression profile in cells. The kit can be used for measuring the relative amounts of CEBPB in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CEBPB.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-CEBPB Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Citations
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Kuruto-Niwa, R; Nakamura, M; et al. Transcriptional regulation by C/EBP alpha and -beta in the expression of the gene for the MRP14 myeloid calcium binding protein. CELL STRUCTURE AND FUNCTION 23:109-118(1998).
Koshfishi, C; Park, HM; et al. Regulation of expression of the mouse adiponectin gene by the C/EBP family via a novel enhancer region. GENE 424:141-146(2008).