CD44var (v6) ELISA Kit (DEIABL329)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Summary
Documentation

Size

96T

Sample

serum, plasma, urine, cell culture supernatant

Species Reactivity

Human

Intended Use

The human CD44var (v6) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human CD44var (v6). The human CD44var (v6) ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Contents of Kit

Microwell Plate: 1 plate
HRP-Conjugate: 1 x 10 uL
Standard: 2 x
Assay Buffer Concentrate 20x: 1 x 5 mL
Wash Buffer Concentrate 20x: 1 x 50 mL
Sample Diluent: 1 x 50 mL
Substrate Solution: 1 x 15 mL
Stop Solution: 1 x 15 mL
Adhesive Films: 2 x

Storage

2-8°C

Precision

Detection Range

0.32 - 10 ng/mL

Sensitivity

0.05 ng/m

General Description

Alternative splicing of the mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). All CD44 isoforms are variably glycosylated. In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed, the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on activated lymphocytes and macrophages (exon v6).
The sCD44var(v6) ELISA detects all circulating CD44 isoforms comprising the sCD44var(v6) sequences.
Determination of sCD44var(v6) will provide more detailed insight into different pathological modifications during cancer and other diseases.

Standard Curve