Human sCD44 ELISA Kit

Name: Human sCD44 ELISA Kit
SKU: DEIA2072
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, tissue homogenates and other biological fluids

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For quantitative detection of CD44 in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

Storage

2-8°C for 6 months

Performance Characteristics

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% within the expiration date under appropriate storage condition.

To minimize extra influence on performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is strongly suggested that the same operator performs the whole assay from the beginning to the end.

Precision

Intra-Assay CV%: <10%
Inter-Assay CV%: <12%

Detection Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard Curve

Citations

Have you cited DEIA2072 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Authors: Rocha Neto, J. B. M.; Gomes Neto, R. J.; Bataglioli, R. A.; Taketa, T. B.; Pimentel, S. B.; Baratti, M. O.; Costa, C. A. R.; Carvalho, H. F.; Beppu, M. M.

Abstract

This paper explores different film assembly conditions of the polyelectrolyte solutions of hyaluronan (HA) and chitosan (CHI), as well as both substrate and cell surface modifications, to investigate PC3 cells adhesion properties. UV-Visible, AFM-IR and Zeta potential techniques indicate that the solution ionic strength is a relevant parameter to modulate the free carboxylic groups of HA on the film surface. In addition, capacitive coupling measurements suggest that assembly conditions that favor surface charge mobility inhibit cell adhesion due to polymer rearrangements that support non-specific electrostatic interactions of positively charged CHI residues and the negatively charged cell moieties, rather than specific CD44-hyaluronan interactions. Moreover, the PC3 cells treatment with hyaluronidase and anti-CD44 antibody also highlighted the importance of CD44 binding site availability on the tumor cell adhesion properties. Finally, the conjugation of wheat germ agglutinin on the film surface proved to be a suitable strategy to boost the PC3 cell adhesion properties. Our results reveal the remarkable capacity of HA/CHI films to modulate cell-substrate properties, which pave the road for the development of surfaces suitable for several applications based on biosensing. (c) 2020 Elsevier B.V. All rights reserved.

Parietal epithelial cell differentiation to a podocyte fate in the aged mouse kidney

AGING-US

Authors: Kaverina, Natalya, V; Eng, Diana G.; Miner, Jeffrey H.; Pippin, Jeffrey W.; Shankland, Stuart J.

Abstract

Healthy aging is typified by a progressive and absolute loss of podocytes over the lifespan of animals and humans. To test the hypothesis that a subset of glomerular parietal epithelial cell (PEC) progenitors transition to a podocyte fate with aging, dual reporter PEC-rtTA/LC1/tdTomato/Nphs1-FLPo/FRT-EGFP mice were generated. PECs were inducibly labeled with a tdTomato reporter, and podocytes were constitutively labeled with an EGFP reporter. With advancing age (14 and 24 months) glomeruli in the juxta-medullary cortex (JMC) were more severely injured than those in the outer cortex (OC). In aged mice (24m), injured glomeruli with lower podocyte number (41% decrease), showed more PEC migration and differentiation to a podocyte fate than mildly injured or healthy glomeruli. PECs differentiated to a podocyte fate had ultrastructural features of podocytes and co-expressed the podocyte markers podocin, nephrin, p57 and VEGF164, but not markers of mesangial (Perlecan) or endothelial (ERG) cells. PECs differentiated to a podocyte fate did not express CD44, a marker of PEC activation. Taken together, we demonstrate that a subpopulation of PECs differentiate to a podocyte fate predominantly in injured glomeruli in mice of advanced age.