WB: A dilution 1:2000 of the antibody was an effective probe for 1ug of unreduced Botulinum Type A holotoxin. Direct ELISA: A dilution of 1:15000 was sufficient to detect a 2 ug coating of Botulinum Meurotoxin Type A. We recommend the following for sandwich ELISA (Capture - Detection): CABT-B1973 - CABT-B1972 For example,a 1 μg coating of CABT-B1973 followed by exposure to BoNT/A and a 1:1000 dilution of CABT-B1972 gives a midpoint at approximately 40 ng/ml. As low as 1 ng/ml of antigen is readily detected using a standard chromogenic substrate.
Target
Alternative Names
Clostridium botulinum Neurotoxin
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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
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Certificate of Analysis
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