Magic™ Anti-Botulinum Neurotoxin, Type A polyclonal antibody (CABT-B1972)

Chicken Anti-Botulinum Neurotoxin, Type A polyclonal antibody for WB, ELISA, sELISA

Specifications


Host Species
Chicken
Antibody Isotype
IgY
Species Reactivity
C. botulinum
Immunogen
Heavy chain domain of Botulinum Neurotoxin Type A
Conjugate
Unconjugated

Applications


Application Notes
WB: A dilution 1:2000 of the antibody was an effective probe for 1ug of unreduced Botulinum Type A holotoxin.
Direct ELISA: A dilution of 1:15000 was sufficient to detect a 2 ug coating of Botulinum Meurotoxin Type A.
We recommend the following for sandwich ELISA (Capture - Detection):
CABT-B1973 - CABT-B1972
For example,a 1 μg coating of CABT-B1973 followed by exposure to BoNT/A and a 1:1000 dilution of CABT-B1972 gives a midpoint at approximately 40 ng/ml. As low as 1 ng/ml of antigen is readily detected using a standard chromogenic substrate.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
Clostridium botulinum Neurotoxin

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Torii, Y; Shinmura, M; et al. Differences in immunological responses of polyclonal botulinum A1 and A2 antitoxin against A1 and A2 toxin. TOXICON 73:9-16(2013).
Mahrhold, S; Strotmeier, J; et al. Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E. BIOCHEMICAL JOURNAL 453:37-47(2013).

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