Anti-di/trimethyl-Lysine polyclonal antibody (DPABT-H20111)

Rabbit anti-Human di/trimethyl-Lysine polyclonal antibody for Dot, WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
KLH-conjugated, synthetic peptide containing the sequence (...AR[me2K]ST...) in which [me2K] corresponds to dimethyl-lysine 9 of human histone H3.
Conjugate
Unconjugated

Applications


Application Notes
Western Blot: A 1:5;000-1:1 0,000 dilution detected di/trimethylated Histone H3 in acid extracts from HeLa cells. No detection of unmodified recombinant histone H3 was observed.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
Dimethylysine antibody; Methyl lysine antibody; N epsilon dimethyl lysine antibody; N epsilon mono methyllysine antibody; N epsilon trimethyl lysine antibody; N(epsilon) trimethyl lysine antibody

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Agarwal, P; Bertozzi, CR; et al. Site-Specific Antibody-Drug Conjugates: The Nexus of Biciorthogonal Chemistry, Protein Engineering, and Drug Development. BIOCONJUGATE CHEMISTRY 26:176-192(2015).
Zhang, L; Li, MM; et al. Essential roles of insulin, AMPK signaling and lysyl and prolyl hydroxylases in the biosynthesis and multimerization of adiponectin. MOLECULAR AND CELLULAR ENDOCRINOLOGY 399:164-177(2015).

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