Anti-ZAP70 monoclonal antibody, clone 2F8.3 [FITC]

Antibody Isotype

IgG1

Clone

2F8.3

Species Reactivity

Human; Mouse

Immunogen

A synthetic peptide (conjugated with KLH) corresponding to amino acids 282-307 of human ZAP70.

Conjugate

Application Notes

Flow Cytometry (10 μl reagent/100 μl of whole blood or 10⁶ cells) & The optimal working dilution should be determined by the end user.

Alternative Names

ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; SRK, zeta chain (TCR) associated protein kinase (70 kD); tyrosine-protein kinase ZAP-70; STD; ZAP 70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protei

Entrez Gene ID

UniProt ID

Antigen Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Pathway

Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Generation of second messenger molecules, organism-specific biosystem; Immune System, organism-specific biosystem; Inflammatory Response Pathway, organism-specific biosystem; Natural killer cell mediated cytotoxicity, organism-specific biosystem; Natural killer cell mediated cytotoxicity, conserved biosystem;