Specificity: WWOX Antibody detects endogenous levels of total WWOX.
Host: Rabbit
Application: ELISA
Species Reactivity: Human, Mouse
Summary
Specifications
Antibody Isotype
IgG
Species Reactivity
Human; Mouse
Immunogen
A synthesized peptide derived from human WWOX.
Conjugate
Unconjugated
Applications
Application Notes
ELISA 1:20000-1:40000
General Notes
This product (Catalog No. DPABH-21671) is a rabbit-derived polyclonal antibody raised against human WWOX. This antibody exhibits cross-reactivity with WWOX from mouse. It has been affinity purified by Protein A. This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. DPABH-21671 is suitable for use in ELISA 1 (20000-1:40000) application. DPABH-21671 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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References
WWOX modulates the ATR-mediated DNA damage checkpoint response
ONCOTARGET
Authors: Abu-Odeh, Mohammad; Hereema, Nyla A.; Aqeilan, Rami I.
For many decades genomic instability is considered one of the hallmarks of cancer. Role of the tumor suppressor WWOX (WW domain-containing oxidoreductase) in DNA damage response upon double strand breaks has been recently revealed. Here we demonstrate unforeseen functions for WWOX upon DNA single strand breaks (SSBs) checkpoint activation. We found that WWOX levels are induced following SSBs and accumulate in the nucleus. WWOX deficiency is associated with reduced activation of ataxia telangiectasia and Rad3-related protein (ATR) checkpoint proteins and increased chromosomal breaks. At the molecular level, we show that upon SSBs WWOX is modified at lysine 274 by ubiquitination mediated by the ubiquitin E3 ligase ITCH and interacts with ataxia telangiectasia-mutated (ATM). Interestingly, ATM inhibition was associated with reduced activation of ATR checkpoint proteins suggesting that WWOX manipulation of ATR checkpoint proteins is ATM-dependent. Taken together, the present findings indicate that WWOX plays a key role in ATR checkpoint activation, while its absence might facilitate genomic instability.
The biological implications of estrogen receptor status and WWOX gene in breast cancer
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Authors: Pospiech, Karolina; Nowakowska, Magdalena; Pluciennik, Elzbieta; Kosla, Katarzyna; Baryla, Izabela; Styczen-Binkowska, Ewa; Bednarek, Andrzej K.
COA
Publication 
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