Anti-VSV-N Hybridoma [21H5] (CSC-H0002LN)

This hybridoma produces mAbs against VSV-N

General Information


Fusion Species
Mouse X Human Transfectoma
Clone
21H5
Cell Line Description
This hybridoma constitutively produces a monoclonal antibody against an epitope in the N protein.
Format
Frozen
Growth Properties
Adherent

Culture Method


Complete Growth Medium
DMEM, supplemented with 10% FBS
Subculturing
Split rate:1:4, 3 times per week. Cells form a confluent monolayer. If grown in a flask, agitation should be enough to dislodge cells.
Storage
Liquid nitrogen vapor phase
Freezing medium: to complete growth medium, add 10%(v/v) DMSO

Target


Introduction
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The N protein (or nucleocapsid protein) tightly binds viral RNA. This activity of the N protein protects the viral genome from cellular sensors and nuclease degradation. Additionally, accumulation of the N protein during VSV replication results in a switch from viral gene transcription to genome replication.

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