Anti-VCP monoclonal antibody, clone 6

Antibody Isotype

IgG2a

Clone

6

Species Reactivity

Mouse; Rat; Sheep; Cow; Human

Immunogen

Synthetic peptide corresponding to Human VCP aa 792-806.Sequence: GGSVYTEDNDDDLYG

Conjugate

Application Notes

IHC-P: 1/500; IHC-Fr: 1/500; Flow Cyt: 1/400; ICC/IF: 1/20 - 1/200; WB: 1/2000.

Alternative Names

VCP; valosin containing protein; transitional endoplasmic reticulum ATPase; IBMPFD; p97; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit; TERA; ALS14; MGC8560; MGC131997; MGC148092;

Entrez Gene ID

UniProt ID

Antigen Description

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.

Pathway

DNA Repair, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; HRD1/SEL1 ERAD complex, organism-specific biosystem; HRD1/SEL1 ERAD complex, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Legionellosis, organism-specific biosystem; Legionellosis, conserved biosystem;