Anti-TERF2 monoclonal antibody, clone 4I7C5

Antibody Isotype

IgG1

Clone

4I7C5

Species Reactivity

Human

Immunogen

Recombinant fragment corresponding to Human TRF2 aa 324-500 (C terminal). Expressed in E. Coli.Sequence: PALKNKRPRKDENESSAPADGEGGSELQPKNKRMTISRLVLEEDSQSTEP SAGLNSSQEAASAPPSKPTVLNQPLPGEKNPKVPKGKWNSSNGVEEKETW VEEDELFQVQAAPDEDSTTNITKKQKWTVEESEWVKAGVQKYGEGNWA

Conjugate

Application Notes

WB: 1/500 - 1/2000; IHC-P: 1/200 - 1/1000;

Alternative Names

TERF2; telomeric repeat binding factor 2; TRBF2; telomeric repeat-binding factor 2; TRF2; telomeric DNA-binding protein; TTAGGG repeat-binding factor 2; telomeric repeat binding protein 2;

Entrez Gene ID

UniProt ID

Antigen Description

Binds the telomeric double-stranded 5-TTAGGG-3 repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5-TTAGGG-3 repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3 single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Pathway

Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Synapsis, organism-specific biosystem; Packaging Of Telomere Ends, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem; Shelterin complex, organism-specific biosystem;