Rabbit Anti-Human TDG (a.a. 1-410) Polyclonal antibody

Supersaturation of total dissolved gas (TDG) is caused by high dam discharge, sudden increases in water temperature and extra photosynthesis of aquatic plants, which may lead to gas bubble disease and even cause mortality of fish. It is imperative to explore treatment measures to mitigate the negative impact of supersaturated TDG for the protection of aquatic organisms. Based on the knowledge of the promotion effect of the aeration bubbles on the mass transfer rate between gas-liquid interfaces, a series of experiments in an aeration column were carried out under different aeration conditions to explore the promotion effect of aeration on the dissipation process of supersaturated total dissolved gas. A quantitative relationship between the dissipation coefficient of supersaturated TDG and the aeration conditions, such as gas flow rate, water depth and pore size, was established. Within the variation range of the aeration conditions, the dissipation coefficient exhibits an exponential increase with the gas flow rate. For a certain gas flow rate, the dissipation coefficient decreases with increasing water depth and diffuser pore size. The exploration of the mass transfer of supersaturated TDG in the aeration tank column provides insight into the quantitative effect of aeration on the dissipation of TDG. This would be useful guidance for further research on the mitigation measures of supersaturated TDG. (C) 2016 Elsevier B.V. All rights reserved.

Traumatic brain injury (TBI) has a high prevalence in our society and often leads to morbidity and mortality. TBI also occurs frequently in a military setting where exposure to blast waves is common. Abnormal gene expression involved with oxidative stress, inflammation and neuronal apoptosis has been well documented following blast induced neurotrauma (BINT). Altered epigenetic transcriptional regulation through DNA methylation has been implicated in the pathology of the injury. Imbalance between DNA methylation and DNA demethylation may lead to altered methylation patterns and subsequent changes in gene transcription. DNA methyltransferase enzymes (DNMT1, DNMT3a, and DNMT3b) are responsible for the addition of methyl groups to DNA, DNA methylation. Whereas the combined function of ten-eleven translocation enzymes (TET1, TET2, and TET3) and thymine-DNA glycosylase (TDG) result in the removal of methyl groups from DNA, DNA demethylation. We used an established rodent model of BINT to assess changes in DNA methylation and demethylation enzymes following injury. Three different blast overpressures were investigated (10, 17 and 23 psi). Gene expression was investigated in the prefrontal cortex and hippocampus two weeks following injury. We observed DNMT, TET and TDG expression changes between pressure groups and brain regions. The hippocampus was more vulnerable to enzyme expression changes than the prefrontal cortex, which correlated with aberrant DNA methylation. A significant negative correlation was found between global DNA methylation and the magnitude of blast overpressure exposure. Through transcriptional regulation, altered DNA methylation patterns may offer insight into the characteristic outcomes associated with the injury pathology including inflammation, oxidative stress and apoptosis. As such, these enzymes may be important targets to future therapeutic intervention strategies. Published by Elsevier Ireland Ltd.