Anti-SMC1A monoclonal antibody (DCABH-192)

Rabbit Anti-SMC1A monoclonal antibody for WB, ICC/IF, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TO31-38
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:100-1:500; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of SMC1 on different lysates using anti-SMC1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3; Lane 2: Hela
Fig2: ICC staining SMC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining SMC1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining SMC1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SMC1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC1 antibody. Counter stained with hematoxylin.

Target


Alternative Names
CDLS2; DKFZp686L19178; DXhXs423e; DXS423E; KIAA0178; MGC138332

Citations


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References


Mu, JJ; Wang, Y; et al. A proteomic analysis of ataxia telangiectasia-mutated (ATM)/ATM-Rad3-related (ATR) substrates identifies the ubiquitin-proteasome system as a regulator for DNA damage checkpoints. JOURNAL OF BIOLOGICAL CHEMISTRY 282:17330-17334(2007).
Sakai, C; Konno, F; et al. Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O-hubbsi. CHROMOSOME RESEARCH 15:697-709(2007).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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