Anti-SMAD4 monoclonal antibody, clone 5H2D7

Antibody Isotype

IgG1

Clone

5H2D7

Species Reactivity

Mouse; Human

Immunogen

Purified recombinant fragment of Human Smad4 expressed in E. Coli.

Conjugate

Application Notes

WB: 1/500 - 1/2000; ELISA: 1/10000; Flow Cyt: 1/200 - 1/400; ICC/IF: 1/200 - 1/1000; IHC-P: 1/200 - 1/1000;

Alternative Names

SMAD4; SMAD family member 4; MAD, mothers against decapentaplegic homolog 4 (Drosophila) , MADH4, SMAD, mothers against DPP homolog 4 (Drosophila); mothers against decapentaplegic homolog 4; DPC4; MAD homolog 4; SMAD, mothers against DPP homolog 4; delet

Entrez Gene ID

UniProt ID

Antigen Description

Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor.

Pathway

ALK1 signaling events, organism-specific biosystem; ALK2 signaling events, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMP Signalling Pathway, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem;