Anti-RHEB (mutation Y35N) Monoclonal Antibody, clone XH113

Clone

XH113

Species Reactivity

Human

Immunogen

A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.

Conjugate

Application Notes

ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Entrez Gene ID

Antigen Description

RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.

Pathway

AMPK signaling pathway; Choline metabolism in cancer; Energy dependent regulation of mTOR by LKB1-AMPK;