Anti-RAD50 monoclonal antibody, clone FQS4577(3)

Antibody Isotype

IgG

Clone

FQS4577(3)

Species Reactivity

Mouse; Human

Immunogen

A synthetic peptide, corresponding to residues in Human Rad50.

Conjugate

Application Notes

WB: 1/1000 - 1/10000.

Alternative Names

RAD50; RAD50 homolog (S. cerevisiae); RAD50 (S. cerevisiae) homolog; DNA repair protein RAD50; hRad50; RAD50 2; NBSLD; RAD502;

Entrez Gene ID

UniProt ID

Antigen Description

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3-5 exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Pathway

Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Homologous Recombination Repair, organism-specific biosystem; Homologous recombination, organism-specific biosystem;