Anti-PMS2 monoclonal antibody, clone 3H6

Antibody Isotype

IgG2b

Clone

3H6

Species Reactivity

Human

Immunogen

Recombinant full length protein corresponding to Human PMS2 aa 1-862. produced in HEK293T cell.Sequence: MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAG ATNIDLKLKDYGVDLIEVSD NGCGVEEENFEGLTLKHHTSKIQEFADL TQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHN GKIIQK

Conjugate

Application Notes

WB: 1/500; IHC-P: 1/150;

Alternative Names

PMS2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMSL2, postmeiotic segregation increased (S. cerevisiae) 2; mismatch repair endonuclease PMS2; H_DJ0042M02.9; HNPCC4; PMS1 protein homolog 2; DNA mismatch repair protein PMS2; PMSL2; PMS2CL;

Entrez Gene ID

UniProt ID

Antigen Description

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Pathway

BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Mismatch repair, organism-specific biosystem; Mismatch repair, conserved biosystem;