Recombinant funsion protein containing a sequence corresponding to amino acids 120-280 of human PAK4 (NP_001014831.1).
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500 - 1:2000
Images
Western blot analysis of extracts of various cell lines, using PAK4 antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
Target
Alternative Names
PAK4; p21 protein (Cdc42/Rac)-activated kinase 4; serine/threonine-protein kinase PAK 4; PAK-4; p21-activated kinase 4; p21(CDKN1A)-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs;
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
Citations
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Andersen, JN; Sathyanarayanan, S; et al. Pathway-Based Identification of Biomarkers for Targeted Therapeutics: Personalized Oncology with PI3K Pathway Inhibitors. SCIENCE TRANSLATIONAL MEDICINE 2:-(2010).
Callow, MG; Clairvoyant, F; et al. Requirement for PAK4 in the anchorage-independent growth of human cancer cell lines. JOURNAL OF BIOLOGICAL CHEMISTRY 277:550-558(2002).
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Rabbit Anti-PAK4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PAK4 antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
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