Anti-ORC6 (aa 191-252) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Synthetic peptide, corresponding to a region within C terminal amino acids 191-252 of Human ORC6 (Uniprot ID: Q9Y5N6).

Conjugate

Application Notes

WB: 1/500 - 1/3000; IHC-P: 1/100 - 1/1000.

Alternative Names

ORC6; origin recognition complex, subunit 6; ORC6L, origin recognition complex, subunit 6 homolog like (yeast); origin recognition complex, subunit 6 like (yeast); origin recognition complex subunit 6; ORC6L;

Entrez Gene ID

UniProt ID

Antigen Description

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC6L is a subunit of the ORC complex. It has been shown that ORC6L and and ORC1L are loosely associated with the core complex consisting of ORC2L, 3L, 4L and 5L. Gene silencing studies with small interfering RNA demonstrated that ORC6L plays an essential role in coordinating chromosome replication and segregation with cytokinesis.

Pathway

Activation of ATR in response to replication stress; Activation of the pre-replicative complex; Assembly of the ORC complex at the origin of replication; Assembly of the pre-replicative complex; CDC6 association with the ORC:origin complex; CDT1 associati