General Notes
This product (Catalog No. DPABH-16236) is a rabbit-derived polyclonal antibody raised against human NFKBIA, targeting the amino acid region 67–302 of the protein. This antibody exhibits cross-reactivity with Human NFKBIA from mouse and rat. It has been affinity purified by Protein A. NFKBIA (NF-κB inhibitor alpha) encodes IκBα, a key negative regulator of the NF-κB signaling pathway. IκBα binds to NF-κB transcription factor complexes (most commonly the p65/RelA–p50 heterodimer) in the cytoplasm, masking their nuclear localization signals and preventing transcriptional activation of target genes. Upon stimulation by pro-inflammatory cytokines, microbial products, or stress signals, IκBα is phosphorylated by the IKK complex, leading to its ubiquitination and proteasomal degradation, which releases NF-κB to translocate into the nucleus and drive expression of genes involved in inflammation, immune responses, cell survival, and proliferation. Tight regulation of NFKBIA is essential for maintaining immune homeostasis, and mutations or dysregulation of this gene have been associated with chronic inflammatory diseases, immunodeficiency, and various cancers.
DPABH-16236 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-16236 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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