GeneralInformation
Immunogen
Activated mouse macrophages
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Cell Line Description
Animals were immunized with activated mouse macrophages. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts weakly with resident mouse peritoneal macrophages and strongly with mouse macrophages that have been activated by treatment with Macrophage Activating Factor (MAF) or thioglycollate. It does not react with rat macrophages nor with a panel of mouse macrophage, monocyte or lymphoid cell lines.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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Lauvsnes, MB; Beyer, MK; et al. Association of Hippocampal Atrophy With Cerebrospinal Fluid Antibodies Against the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in Patients With Systemic Lupus Erythematosus and Patients With Primary Sjogren's Syndrome. ARTHRITIS & RHEUMATOLOGY 66:3387-3394(2014).
Markham, NO; Doll, CA; et al. DIPA-family coiled-coils bind conserved isoform-specific head domain of p120-catenin family: potential roles in hydrocephalus and heterotopia. MOLECULAR BIOLOGY OF THE CELL 25:2592-2603(2014).
COA
Certificate of Analysis
