Anti-MBP monoclonal antibody, clone 8H8

Introgression from wild relatives is a valuable source of novel allelic diversity for breeding. We investigated the genomic patterns of introgression fromAegilops tauschii, the diploid ancestor of the wheat D genome, into winter wheat (Triticum aestivum) cultivars. The population of 351 BC1F3:(5)lines was selected based on phenology from crosses between six hexaploid wheat lines and 21 wheat-Ae. tauschiioctoploids. SNP markers developed for this population and a diverse panel of 116Ae. tauschiiaccessions by complexity-reduced genome sequencing were used to detect introgression based on the identity-by-descent analysis. Overall, introgression frequency positively correlated with recombination rate, with a high incidence of introgression at the ends of chromosomes and low in the pericentromeric regions, and was negatively related to sequence divergence between the parental genomes. Reduced introgression in the pericentromeric low-recombining regions spans nearly 2/3 of each chromosome arm, suggestive of the polygenic nature of introgression barriers that could be associated with multilocus negative epistasis between the alleles of wild and cultivated wheat. On the contrary, negative selection against the wild allele ofTg, controlling free-threshing trait and located in the high-recombining chromosomal region, led to reduced introgression only within similar to 10 Mbp region aroundTg. These results are consistent with the effect of selection on linked variation described by the Hill-Robertson effect, and offer insights into the introgression population development for crop improvement to maximize retention of introgressed diversity across entire genome.

The effect of Mesona blumes polysaccharide (MBP) on the microstructure and gel properties of glucono-delta-lactone-induced soy protein isolate (SPI) gels was evaluated by texture, water holding capacity, rheology, and microstructure analysis. The results showed that the apparent viscosities and storage modulus (G') of the SPI-MBP gels were increased as the MBP concentration increased. The addition of MBP promoted the water holding capacity (WHC) and gel strength of SPI-MBP gels. Scanning electron microscopy (SEM) showed that, in the presence of MBP, the surface of mixed gels became smooth and the structure became dense. Additionally, the zeta potential and interactions results indicated that electrostatic and hydrophobic interactions played an important role in maintaining the three-dimensional structure of SPI-MBP gels. In conclusion, the results of this study suggest that MBP is desirable for SPI-MBP gels as a gelling agent.