GeneralInformation
Immunogen
Recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362).
Application Notes
WB, ELISA, ICC, and IHC of the human LH/hCG receptor.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Target
Synonyms
LHCGR; luteinizing hormone/choriogonadotropin receptor; HHG, hypergonadotropic hypogonadism; lutropin-choriogonadotropic hormone receptor; LCGR; LGR2; LHR; ULG5; hypergonadotropic hypogonadism; lutropin/choriogonadotropin receptor; HHG; LHRHR; LSH-R; LH/CGR; LH/CG-R; FLJ41504;
Citations
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Marelli, BE; Diaz, PU; et al. mRNA expression pattern of gonadotropin receptors in bovine follicular cysts. REPRODUCTIVE BIOLOGY 14:276-281(2014).
Schaarschmidt, J; Huth, S; et al. Influence of the Hinge Region and Its Adjacent Domains on Binding and Signaling Patterns of the Thyrotropin and Follitropin Receptor. PLOS ONE 9:-(2014).
COA
Certificate of Analysis
