GeneralInformation
Immunogen
Recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362). The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.
Application Notes
WB, ELISA, ICC, and IHC of the human LH/hCG receptor.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Target
Synonyms
LHCGR; luteinizing hormone/choriogonadotropin receptor; HHG, hypergonadotropic hypogonadism; lutropin-choriogonadotropic hormone receptor; LCGR; LGR2; LHR; ULG5; hypergonadotropic hypogonadism; lutropin/choriogonadotropin receptor; HHG; LHRHR; LSH-R; LH/CGR; LH/CG-R; FLJ41504;
Citations
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Liu, KC; Lin, SW; et al. Differential Regulation of Gonadotropin Receptors (fshr and lhcgr) by Estradiol in the Zebrafish Ovary Involves Nuclear Estrogen Receptors That Are Likely Located on the Plasma Membrane. ENDOCRINOLOGY 152:4418-4430(2011).
Kossack, N; Terwort, N; et al. A combined approach facilitates the reliable detection of human spermatogonia in vitro. HUMAN REPRODUCTION 28:3012-3025(2013).
COA
Certificate of Analysis
