Anti-KPNA2 monoclonal antibody, clone FQS22828

Name: Anti-KPNA2 monoclonal antibody, clone FQS22828
SKU: DCABH-5854

Rabbit anti-Human KPNA2 monoclonal antibody for WB

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COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Clone

FQS22828

Species Reactivity

Human

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KPNA2 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.Database link: P52292

Conjugate

Unconjugated

Applications

Application Notes

WB: 1/1000 - 1/5000;

Target

Alternative Names

KPNA2; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); RCH1; importin subunit alpha-2; IPOA1; QIP2; SRP1alpha; pendulin; SRP1-alpha; RAG cohort 1; importin alpha 1; importin alpha 2; importin-alpha-P1; RAG cohort protein 1; karyopherin subunit alpha

Entrez Gene ID

3838

UniProt ID

P52292

Product Background

Antigen Description

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Pathway

Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; Interferon Signaling, organism-specific biosystem;

Citations

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Documents

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References

Screening several novel biomarkers for predicting the survival of patients with lung adenocarcinoma

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE

Authors: Li, Mingzhu; Wang, Shenyu

Abstract

Background: Increasing evidence has confirmed that long non-coding RNAs (lncRNAs) could regulate protein levels of genes as well as the cellular biological behavior in the competing endogenous RNA (ceRNA) network as miRNA sponges. Our study aimed to explore the role and regulatory mechanisms of lncRNA-mediated ceRNA network in lung adenocarcinomas (LUAD), as well as their potential to predict the prognosis of LUAD. Methods: A total of 568 samples with LUAD were downloaded from The Cancer Genome Atlas (TCGA). Differentially expressed IncRNAs (DElncRNAs), miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) were identified with EdgeR package in R according to vertical bar log fold change vertical bar > 2 and a corrected p-value < 0.05. DElncRNA-DEmiRNA pairs were predicted by the online database of miRcode, while DEmRNA-DEmiRNA pairs were predicted through "miRDB", "miRTarBase" and "TargetScan". And the ceRNA network was visualized through Cytoscape 3.6. After calculating the degrees of lncRNA nodes, we constructed the ceRNA sub-network. Finally, overall survival analysis was utilized to assess prognostic performance of differentially expressed genes in sub-network. Results: 1503 DElncRNAs, 118 DEmiRNAs and 2501 DEmRNAs were identified associated with LUAD. And the ceRNA network was constructed containing 120 lncRNA nodes, 23 miRNA nodes and 39 mRNA nodes. 5 lncRNAs including MEG3, AP002478.1, LINC00461, HOTTIP and MUC2 (degree > 12) were selected to build the sub-network. And two lncRNAs (AP002478.1 and MUC2) and seven mRNAs (E2F7, KPNA2, CEP55, HOXA10, CCNE1, CHEK1 and CLSPN) in the sub-network had poor prognosis according to overall survival analysis. Conclusion: MUC2 and AP002478.1 could become potential prognostic biomarkers for patients with LUAD. Based on the ceRNA hypothesis, we predicted that AP002478.1-induced AP002478.1-hsa-mir144-HOXA10/KPNA2 and MUC2-hsa-mir-195-CCNE1/CEP55/CHEK1/CLSPN/E2F7/HOXA10 pathways are associated with LUAD and will be investigated for further experimental confirmation.

Karyopherin Alpha2 Is Essential for rRNA Transcription and Protein Synthesis in Proliferative Keratinocytes

PLOS ONE

Authors: Umegaki-Arao, Noriko; Tamai, Katsuto; Nimura, Keisuke; Serada, Satoshi; Naka, Tetsuji; Nakano, Hajime; Katayama, Ichiro

Abstract

Karyopherin proteins mediate nucleocytoplasmic trafficking and are critical for protein and RNA subcellular localization. Recent studies suggest KPNA2 expression is induced in tumor cells and is strongly associated with prognosis, although the precise roles and mechanisms of KPNA2 overexpression in proliferative disorders have not been defined. We found that KPNA2 expression is induced in various proliferative disorders of the skin such as psoriasis, Bowen's disease, actinic keratosis, squamous cell carcinoma, Paget's disease, Merkel cell carcinoma, and mycosis fungoides. siRNA-mediated KPNA suppression revealed that KPNA2 is essential for significant suppression of HaCaT proliferation under starvation conditions. Ribosomal RNA transcription and protein synthesis were suppressed by starvation combined with knockdown of KPNA (including KPNA2) expression. KPNA2 localized to the nucleolus and interacted with proteins associated with mRNA processing, ribonucleoprotein complex biogenesis, chromatin modification, and transcription, as demonstrated by tandem affinity purification and mass spectrometry. KPNA2 may be an important promoter of ribosomal RNA and protein synthesis in tumor cells.