UNRAVELLING THE ROLE OF IL33/ST2L AXIS ON SUSTAINED IFN-A PRODUCTION IN SYSTEMIC LUPUS ERYTHEMATOSUS
ANNALS OF THE RHEUMATIC DISEASES
Authors: Georgakis, Spiridon; Papadaki, Garyfallia; Gergianaki, Irene; Sidiropoulos, Prodromos; Verginis, Panagiotis; Gkirtzimanaki, Aikaterini; Bertsias, George
Abstract
Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
MEDIATORS OF INFLAMMATION
Authors: Santana de Oliveira, Priscilla Stela; Pereira, Michelly Cristiny; Silva de Paula, Simao Kalebe; Andrade Lima, Emerson Vasconcelos; de Andrade Lima, Mariana Modesto; de Arruda, Rodrigo Gomes; Mendes de Oliveira, Wagner Luis; Branco Pinto Duarte, Angela Luzia; Pitta, Ivan da Rocha; Melo Barreto Rego, Moacyr Jesus; da Rocha Pitta, Maira Galdino
Abstract
Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity.
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