Anti-IL12A monoclonal antibody, clone CC326

The development of vaccines for aquaculture has been an important milestone in providing a continuous and sustainable production. Most of the vaccines currently on the market for aquaculture include oil as adjuvant. Nevertheless, several studies reported an occurrence of side effects after their use in farmed fish. As a result, there is a need for new and improved adjuvants that can stimulate the immune system while causing as few side-effects as possible. Hemocyanins are versatile macromolecules with strong immunogenic and immunomodulatory properties. Due to these characteristics, hemocyanin from Concholepas concholepas (CCH) has been biochemically characterized and evaluated as vaccine adjuvant in mice and humans. Francisellosis is a chronic granulomatous disease, which can result in high mortality depending on the host. The disease is caused by the facultative intracellular Gram-negative bacteria Francisella noatunensis, which remains an unsolved problem for the aquaculture, as no efficient vaccines are available. The aim of the present work was to investigate the immunoregulatory properties of CCH against francisellosis in an experimental zebrafish model. When immunized with CCH, zebrafish were protected from subsequent challenge with a lethal dose of Francisella noatunensis subsp. orientatis. Subsequently the mRNA expression levels of several immune-related genes were studied, including mhcii, il12a, tnf alpha and ifng1-1. Taken together, the data report the immunoregulatory properties of CCH and its potential use as a vaccine adjuvant for aquaculture. (C) 2017 The Authors. Published by Elsevier Ltd.

Cytokine and cytokine receptor genes, including IL2RA, IL7R and IL12A, are known risk factors for multiple sclerosis (MS). Excitotoxic oligodendroglial death mediated by glutamate receptors contributes to demyelinating reactions. In the present study, we screened 368 single-nucleotide polymorphisms (SNPs) in 55 genes or gene clusters coding for cytokines, cytokine receptors, suppressors of cytokine signaling (SOCS), complement factors and glutamate receptors for association with MS in a Spanish Basque resident population. Top-scoring SNPs were found within or nearby the genes coding for SOCS-1 (P = 0.0005), interleukin-28 receptor, alpha chain (P = 0.0008), oncostatin M receptor (P = 0.002) and interleukin-22 receptor, alpha 2 (IL22RA2; P = 0.003). The SOCS1 rs243324 variant was validated as risk factor for MS in a separate cohort of 3919 MS patients and 4003 controls (combined Cochran-Mantel-Haenszel P = 0.00006; odds ratio (OR) = 1.13; 95% confidence interval (CI) = 1.07-1.20). In addition, the T allele of rs243324 was consistently increased in relapsing-remitting/secondary progressive versus primary-progressive MS patients, in each of the six data sets used in this study (P-CMH = 0.0096; OR = 1.24; 95% Cl 1.05-1.46). The association with SOCS1 appears independent from the chr16MS risk locus CLEC16A. Genes and Immunity (2012) 13, 21-28; doi:10.1038/gene.2011.44; published online 30 June 2011