Monkey Interleukin-12 (p70) ELISA Kit

Name: Monkey Interleukin-12 (p70) ELISA Kit
SKU: DEIA4684
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Cell culture supernatant

Species Reactivity

Monkey

Detection Method

sELISA

Intended Use

The monkey IL-12 ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of monkey Interleukin-12 in cell culture supernatants, monkey serum, plasma or other body fluids. This ELISA specifically recognizes biologically active heterodimeric p70 IL-12. It does not cross react with the p40 antagonist. The monkey IL-12 ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.

Contents of Kit

1. 1 vial (100 μl) Biotin-Conjugate anti-monkey-IL-12 monoclonal antibody
2. 1 vial (150 μl) Streptavidin-HRP
3. 2 vials monkey IL-12 Standard, lyophilized, 500 U/ml upon reconstitution
4. 1 bottle (50 ml) Wash Buffer Concentrate 20× (PBS with 1% Tween 20)
5. 1 vial (5ml) Assay Buffer Concentrate 20× (PBS with 1% Tween 20 and 10 % BSA)
6. 1 bottle (12ml) Sample Diluent (Note: In some cases the Sample Diluent contains insoluble white precipitations which do not interfere with the test performance. Use according to protocol.)
7. 1 vial (15 ml) Substrate Solution
8. 1 vial (12 ml) Stop Solution (1M Phosphoric acid)
9. 1 vial (0.4 ml each) Blue-Dye, Green-Dye, Red-Dye

Storage

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Detection Range

3.9-250 U/ml

Detection Limit

1.6 U/ml

Sensitivity

The limit of detection of monkey IL-12 defined as the analyte concentration resulting in an absorption significantly higher than that of the dilution medium (mean plus two standard deviations) was determined to be less than 2.64 U/ml (mean of 6 independent assays).

General Description

Interleukin-12 (IL-12) is a pleiotropic cytokine, formerly termed cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF), which is produced primarily by stimulated macrophages. It was originally identified as a factor produced by human Epstein-Barr Virus transformed B cell lines. Meanwhile IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen - presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells. IL-12 exerts a variety of biological effects on T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of interferon (IFN) gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-gamma then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN-gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections. Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Chinese sweet tea (Rubus suavissimus) polyphenols attenuate the allergic responses in a Balb/c mouse model of egg allergy

JOURNAL OF FUNCTIONAL FOODS

Authors: Mine, Yoshinori; Majumder, Kaustav; Jin, Yan; Zeng, Yuhan

Abstract

The aim of this study was to evaluate the effects of Rubus suavissimus S. Lee extract against hen egg ovalbumin (OVA)-induced allergic response in mice. BALB/c mice were sensitized with OVA via intraperitoneal injection for four weeks and subsequently administered the different doses of Rubus suavissimus S. Lee extract (0.1 0.5 and 1.0% w/v) in drinking water. We demonstrated that Rubus suavissimus S. Lee extract can attenuate OVA allergic response in mice by inducing immune desensitization through Thl/Th2 immunological modulation, a regulatory response involving the transcription factor FOXP3, induction of a suppressed IL-4, but increased IL-10, IL12a and INF-gamma, enhanced immunoglobulin isotype IgA production, and suppression of histamine and MMCPT-1. These results suggest that Rubus suavissimus S. Lee extract may be a potentially useful candidate for the design of a functional food component in targeting management of food allergy.

Comprehensive evaluation of positional candidates in the IL-18 pathway reveals suggestive associations with schizophrenia and herpes virus seropositivity

AMERICAN JOURNAL OF MEDICAL GENETICS PART B-NEUROPSYCHIATRIC GENETICS

Authors: Shirts, Brian H.; Wood, Joel; Yolken, Robert H.; Nimgaonkar, Vishwajit L.

Abstract

Interactions between genetic variation and environmental factors have been invoked in schizophrenia genesis, but pathways linking them are uncertain. We used a pathway-oriented approach to evaluate six genes mediating IL18 function (IL-18, IL18BP, IL18R1, IL18RAP, IL12B, and IL12A). The first five are also localized to regions previously linked with schizophrenia. Fifty-four representative tag SNPs were selected from comprehensive sequence data and genotyped in 478 patients with schizophrenia/schizoaffective disorder (DSM IV criteria) and 501 unscreened control individuals. Exposure to three herpes viruses previously suggested as risk factors for schizophrenia was estimated simultaneously among the cases. Five SNPs in four genes were associated with schizophrenia, most prominently rs2272127 at IL18RAP (P = 0.0007, odds ratio for C allele 1.49, 95% CI: 1.18-1.87; P = 0.03 following correction for multiple comparisons). Exploratory analysis revealed that rs2272127 was also associated with herpes simplex virus 1 (HSV1) seropositivity in cases (P = 0.04, OR for G allele 1.58, 95% CI: 1.04-2.39). Similar patterns were observed at another correlated SNP (rs11465702, P = 0.005 and 0.006, respectively for associations with schizophrenia and HSV1 seropositivity). We suggest plausible, testable hypotheses linking IL-18 signaling and HSV1 in schizophrenia pathogenesis. (C) 2007 Wiley-Liss, Inc.